Construction of a recombinant adeno-associated virus (rAAV) vector expressing murine interleukin-12 (IL-12)
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چکیده
منابع مشابه
Enhanced Immune Responses of a Hepatitis C Virus core DNA Vaccine by co-Inoculating Interleukin-12 Expressing Vector in Mice
Background: Hepatitis C (HCV) is a worldwide problem without an effective vaccine with more than 170 million chronically infected people worldwide. DNA vaccines expressing antigenic portions of the virus with adjutants have recently been developed as a novel vaccination technology. Objectives: In the present study, a DNA vaccine expressing HCV core protein was developed with IL12 as a genetic a...
متن کاملSuppression of experimental uveitis by a recombinant adeno-associated virus vector encoding interleukin-1 receptor antagonist
PURPOSE To evaluate the potential of gene therapy with a recombinant adeno-associated virus vector encoding the interleukin-1 receptor antagonist gene (rAAV-IL-1Ra) in the treatment of experimental uveitis. METHODS The vitreal cavity of New Zealand white rabbits was injected with rAAV-IL-1Ra (4x10(7) infectious units), and the contralateral eye was injected with the same amount of rAAV-LacZ o...
متن کاملConstruction and Detection of Recombinant Adeno-Associated Virus Co- Expressing PRRSV GP5, M Proteins and shRNA
Porcine reproductive and respiratory syndrome virus (PRRSV) is a severe threat to the swine industry and has caused heavy economic losses worldwide. The currently used inactivated and attenuated virus vaccines have several shortcomings, such as unsafety and low protection rate, so it is urgent to develop a new vaccine. To explore and develop a novel vaccine against PRRSV, a bi-functional recomb...
متن کاملenhanced immune responses of a hepatitis c virus core dna vaccine by co-inoculating interleukin-12 expressing vector in mice
background: hepatitis c (hcv) is a worldwide problem without an effective vaccine with more than 170 million chronically infected people worldwide. dna vaccines expressing antigenic portions of the virus with adjutants have recently been developed as a novel vaccination technology. objectives: in the present study, a dna vaccine expressing hcv core protein was developed with il12 as a genetic a...
متن کاملA new method for recombinant adeno-associated virus vector delivery to murine diaphragm.
Genetically modified mice are important models for evaluation of potential gene therapies for human diseases. However, their small size often precludes the use of clinically feasible methods for vector delivery, therefore, alternative methods must be used. We have developed a gel-based method for delivery of recombinant adeno-associated virus vectors to the mouse diaphragm, an important target ...
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ژورنال
عنوان ژورنال: Cancer Gene Therapy
سال: 2000
ISSN: 0929-1903,1476-5500
DOI: 10.1038/sj.cgt.7700105